Chinese scientists for the first time revealed the expression of mammary stem cell microenvironment factors Rspo1 by estrogen and progesterone regulation, and clever use of hormonal signals upstream micro-environmental factors, to establish a new method of breast stem cells in vitro and amplification.
Hormones affect breast organ development, the body of the number of mammary stem cells and breast cancer development and progression. Previous studies have demonstrated mammary stem cells do not express hormone receptors, and therefore prompted through systemic hormonal signals of local growth factors (microenvironment signal) so that the medium of the breast stem cells play a role. Previous studies have revealed Wnt family of art researcher directly affect mammary stem cell self-renewal microenvironment signals (Zeng and Nusse, Cell Stem Cell, 2010), for the use of micro-environmental factors in vivo expansion of stem cells has accumulated a wealth of experience. However, in vivo Wnt-related molecules which promote stem cell proliferation, whether hormone-mediated effects on stem cells is still a pending issue.
Under the guidance of art researcher who postdoctoral Cai car country found Rspo1 and Wnt4 signaling molecules in adjacent cells and synergy together constitute mammary stem cells in vivo micro-environmental factors in order to promote mammary stem cell self-renewal. Further studies showed that Rspo1 and Wnt4 expression by estrogen and progesterone regulation and established on the basis of this theory using hormones on breast stem cells in vitro amplification and maintain their stem cell characteristics approach. This study demonstrates the important role of hormones in breast stem cell proliferation, new training methods used hormone replacement expensive Wnt purified protein and beneficial method for culturing stem cells in the laboratory popularity for antagonist drug for stem cell hormone Screening for breast cancer provides new ideas and new strategies.
This work was supported by the Chinese Academy of Sciences, National Science and Technology, the National Natural Science Foundation of China, China Postdoctoral surface funds, and funds to support the Shanghai Science and Technology Commission. (Source: Bio help )
Original title: R-spondin1 is a novel hormone mediator for mammary stem Cell self-renewal